Part:BBa_K5300043:Design

PglnK-sgRNA-GFP-tracrRNA-PnifH-gfp

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 2218
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1623
  

Design Notes

When designing regulatory module, we added terminators derived from genome of Sinorhizobium fredii CCBAU45436 to gfp and sgRNA-GFP to ensure that regulation proceeds as expected

Source

Sinorhizobium fredii CCBAU45436, Bradyrhizobium diazoefficiens USDA 110 (Bradyrhizobium japonicum USDA 110), Scytonema hofmannii.